Leukemogenesis: Duration-of-Life Gamma-Irradiation of Young Adult Beagles

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Until 1991 Tom Seed performed one of several Beagle Dog Experiments at Argonne National Laboratory. He treated dogs to external cobalt-60 gamma ray exposure, 22 hrs/d at 75 mGy/d in order to investigate the consequences of duration-of-life exposure at low dose rates on leukaemogenesis.

Gray Book Description

Copied from: GB Gerber, CR Watson, T Sugahara, and S Okada. International Radiobiology Archives of Long-Term Animal Studies I. Descriptions of Participating Institutions and Studies (1996) ([1])

Institution: Argonne National Laboratory, Argonne IL

Scientists: Tom Seed; retired.

Purpose To investigate the consequences of duration-of-life exposure at low dose rates on leukaemogenesis.

Status Terminated Jan 1991; survivors dispersed.

Treatment External cobalt-60 gamma ray exposure, 22 hrs/d, 75 mGy/d. Dogs were irradiated 22 h/d, 7 d/w, in a specially constructed facility. Particular attention was given to dosimetry; all factors contributing to the dose rate and total dose were normalized in the irradiation field by migrating dogs through all positions and orientations with respect to the irradiation source. Control dogs were similarly housed in cages and migrated through positions in the control animal room.

Dosimetry: Radiation was delivered with a cobalt-60 gamma beam apparatus equipped with steel attenuators which were changed every few months to compensate for radioactive decay. Beagles were caged singly in two-tiered fiberglass cages placed at calculated distances from the source; cages were rotated daily to compensate for the propensity of the dog to occupy the rear of the cage. Dose rate at the center of the cage was measured and converted to absorbed dose.

Endpoints evaluation of bone marrow structure and function leading to aplastic anemia, myelogenous leukemia, or protracted survival.

Animal Young adult Beagle dogs



Experimental Groups Not available


Data from this study is available freely from the Beagle Dog archive at northwestern [2] and from the European Radiobiology Archive [3] ( password required ).


Tissues preserved from these studies are stored at the Beagle Dog Archive at Northwestern University. Tissues may be searched for using their website [4].

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